学位

• 修士（工学）(群馬大学)
• 博士（工学）(群馬大学)

経歴

学歴

受賞

• 分野融合研究優秀表彰
• 岡山大学若手トップリサーチャー学長表彰
• 有機合成化学協会企画賞
• 日本化学会第72回春季年会・高分子部門最優秀ポスター発表賞
• 第5回国際超分子化学サマースクール・最優秀ポスター発表賞
• 日本化学会第３回バイオ関連化学合同シンポジウム講演賞

その他

論文

• bioTCIs: Middle-to-Macro Biomolecular Targeted Covalent Inhibitors Possessing Both Semi-Permanent Drug Action and Stringent Target Specificity as Potential Antibody Replacements [DOI] [Web of Scientce]
• Relative Nuclease Resistance of a DNA Aptamer Covalently Conjugated to a Target Protein
• Solvatochromic peptidic binder obtained via extended phage display acts as a fluororeporter for fragment-based drug discovery (FBDD)
• Covalent Biologics：中・高分子型共有結合性薬剤
• 薬剤の中和が可能なアプタマー型共有結合性薬剤の開発
• Inhibition of thrombin activity by a covalent-binding aptamer and reversal by the complementary strand antidote [DOI] [PubMed]
• Medium-firm drug-candidate library of cryptand-like structures on T7 phage: Design and selection of strong binder for Hsp90 [DOI] [PubMed]
• Direct screening of a target-specific covalent binder: stringent regulation of warhead reactivity in a matchmaking environment [DOI] [Othres link]
• Strategic design to create HER2-targeting proteins with target-binding peptides immobilized on a fibronectin type III domain scaffold [DOI] [Web of Scientce]
• Facile and Efficient Chemoenzymatic Semisynthesis of Fc-Fusion Compounds for Half-Life Extension of Pharmaceutical Components. [DOI] [PubMed]
• Mechano-chromic protein–polymer hybrid hydrogel to visualize mechanical strain [DOI] [Othres link]
• Cysteine-reactive small photo-crosslinker possessing caged-fluorescence property: binding-site determination of a combinatorially-selected peptide by fluorescence imaging / tandem mass spectrometry [DOI] [Othres link]
• Fluorescent "keep-on" type pharmacophore obtained from dynamic combinatorial library of Schiff bases
• Combinatorially Screened Peptide as Targeted Covalent Binder: Alteration of Bait-Conjugated Peptide to Reactive Modifier [DOI]
• Combinatorially screened peptide as targeted covalent binder [Web of Scientce]
• Selection of Turning-on Fluorogenic Probe as Protein-Specific Detector Obtained via the 10BASE(d)-T [DOI] [Web of Scientce]
• 人工コアのファージ上での分子進化 [CiNii Research (CiNii Articles)] [CiNii Books] [Othres link] [Othres link]
• F-18-Containing Positron Emission Tomography Probe Conjugation Methodology for Biologics as Specific Binders for Tumors [DOI] [Web of Scientce]
• Chemical and Biological Technology for In Vivo and Molecular Imaging [Web of Scientce]
• Selection of Color-Changing and Intensity-Increasing Fluorogenic Probe as Protein-Specific Indicator Obtained via the 10BASE(d)-T [DOI] [Web of Scientce]
• Unexpectedly fast transfer of positron-emittable artificial substrate into N-terminus of peptide/protein mediated by wild-type L/F-tRNA-protein transferase [DOI] [Web of Scientce]
• Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASE(d)-T on Bacteriophage T7 [DOI] [Web of Scientce]
• Construction of a crown ether-like supramolecular library by conjugation of genetically-encoded peptide linkers displayed on bacteriophage T7 [DOI] [Web of Scientce]
• Gp10 based-thioetherification (10BASEd-T) on a displaying library peptide of bacteriophage T7 [DOI] [PubMed]
• Kinetic analysis of the leucyl/phenylalanyl-tRNA-protein transferase with acceptor peptides possessing different N-terminal penultimate residues [DOI] [Web of Scientce]
• Practical tips for construction of custom peptide libraries and affinity selection by using commercially available phage display cloning systems [DOI]
• Synthesis of a cyclic peptide/protein using the NEXT-A reaction followed by cyclization [DOI] [Web of Scientce]
• Fission yeast Ubrl ubiquitin ligase influences the oxidative stress response via degradation of active Papl bZIP transcription factor in the nucleus
• Introduction of a Highly Photodurable and Common-laser Excitable Fluorescent Amino Acid into a Peptide as a FRET Acceptor for Protease Cleavage Detection [DOI] [Web of Scientce]
• N-Terminal Specific Point-Immobilization of Active Proteins by the One-Pot NEXT-A Method [DOI] [Web of Scientce]
• The NEXT-A (N-terminal extension with transferase and ARS) reaction
• Chemoenzymatic transfer of fluorescent non-natural amino acids to the N terminus of a protein/peptide [DOI] [Web of Scientce]
• Leucyl/phenylalanyl(L/F)-tRNA-protein transferase-mediated aminoacyl transfer of a nonnatural amino acid to the N-terminus of peptides and proteins and subsequent functionalization by bioorthogonal reactions [DOI] [Web of Scientce]
• Design of carrier tRNAs and selection of four-base codons for efficient incorporation of various nonnatural amino acids into proteins in Spodoptera frugiperda 21 (Sf21) insect cell-free translation system [DOI] [Web of Scientce]
• Leucyl/phenylalanyl-tRNA-protein transferase-mediated chemoenzymatic coupling of N-terminal arg/lys units in posttranslationally processed proteins with non-natural amino acids [DOI] [Web of Scientce]
• Expanding the genetic code in a mammalian cell line by the introduction of four-base codon/anticodon pairs [DOI] [Web of Scientce]
• Efficient Incorporation of a Nonnatural Amino Acid into a Protein in an Insect Cell-free Translation System
• Position-specific incorporation of a highly photodurable and blue-laser excitable fluorescent amino acid into proteins for fluorescence sensing [DOI] [Web of Scientce]
• Creation of artificial fluorescence protein in which a novel fluorescent nonnatural amino acid is site-specifically incorporated for new biosensing
• Synthesis of novel luminescent substrates and their incorporation into a protein only at a terminal site via a transglutaminase-catalyzed enzymatic reaction [Web of Scientce]
• Transglutaminase-mediated N- and C-terminal fluorescein labeling of a protein can support the native activity of the modified protein [DOI] [Web of Scientce]
• Small-interfering-RNA expression in cells based on an efficiently constructed Dumbbell-shaped DNA [DOI] [Web of Scientce]
• Enzymatic N- and C- terminal fluorescein labelling of a protein in vitro can support the native activity of the modified protein
• Nature of the Chemical Bond Formed with the Structural Metal Ion at the A9/G10.1 Motif Derived from Hammerhead Ribozymes
• Synthesis and antigen-binding properties of fluorescent labeled camel antibody
• Site-directed incorporation of non-natural amino acid into Streptomyces xylanase
• Synthesis of a novel histidine analogue and its efficient incorporation into a protein in vivo [DOI] [Web of Scientce]
• A direct and efficient synthesis method for dumbbell-shaped linear DNA using PCR in vitro
• Leucyl/Phenylalanyl (L/F)-tRNA-protein transferase-mediated N-terminal specific labelling of a protein in vitro
• Position-specific incorporation of a fluorophore-quencher pair into a single streptavidin through orthogonal four-base codon/anticodon pairs [DOI] [Web of Scientce]
• Highly sensitive fluorescent nonnatural amino acids that can be incorporated into specific positions of streptavidin [DOI] [Web of Scientce]
• A novel fluorescent nonnatural amino acid that can be incorporated into a specific position of streptavidin
• A non-natural amino acid for efficient incorporation into proteins as a sensitive fluorescent probe [Web of Scientce]
• Specific N-terminal biotinylation of a protein in vitro by a chemically modified tRNA(fmet) can support the native activity of the translated protein [Web of Scientce]
• Site-specific cleavage of a protein via introducing a hydroxy acid derivative in the main chain by using four-base codon/anticodon pairs
• A novel immobilization method of an active protein via in vitro N-terminal specific incorporation system of nonnatural amino acids
• Langmuir films of amphiphilic [60]fullerene derivatives [Web of Scientce]
• A chiral Eu3+-thienoyltrifluoroacetone complex on an avidin tetramer: luminescence and CD studies on the supramolecular protein-metal chelate complex [Web of Scientce]
• Photophysical properties of various regioisomers of [60]fullerene-o-quinodimethane bisadducts [Web of Scientce]
• [60]Fullerene (A(1),D-1)-bisadducts: CD spectra of enantiomers and diastereospecific synthesis [Web of Scientce]
• Selective functionalization on [60]fullerene governed by tether length [Web of Scientce]
• Synthesis of polyesters containing the [60]fullerene moiety in the main chain [Web of Scientce]
• [60]フラーレンへのオルトキノジメタン及びその類縁体の付加反応と生成物の性質
• フラーレンの化学の最近の進歩 その1 -[60]フラーレンへのオルトキノジメタンおよびその類縁体の付加反応・生成物の物性
• NOMENCLATURE OF [60]FULLERENE DERIVATIVES BY EDGE LABELING [Web of Scientce]
• PHOTOREDUCTION OF WATER BY THE SYSTEM OF C-60-PLATINUM-METHYLVIOLOGEN [Web of Scientce]

その他業績

• ペプチド型薬剤の共有結合化と血中安定性獲得
• PACIFICHEM2015今昔
• L/F-転移酵素を用いた蛋白質N末端への機能性非天然アミノ酸導入
• カリフォルニア工科大学における研究教育
• 気になった論文
• 非天然アミノ酸の導入による蛋白質の蛍光ラベル法とその応用 [CiNii Research (CiNii Articles)] [CiNii Books] [Othres link]
• Position-specific incorporation of a fluorophore-quencher pair into a single protein through orthogonal 4-base codon/anticodon pairs. [Web of Scientce]
• 活性蛋白質の蛍光標識技術

講演・口頭発表等

• Discovery and characterization of a short peptide possessing a latent warhead undergoing spontaneous intramolecular cyclization in the aqueous environment
• 中分子共有結合薬剤 (bioTCI)：ペプチド型TCIの直接選択とアプタマーのTCI化
• 中分子共有結合薬剤の基礎開発と医工産学連携の実例
• 日本の学会の現状：全2037の国内学会の定量解析と成功／失敗事例
• 中分子共有結合薬剤
• Design, Selection, and Engineering of Targeted Hybrid-Middle Molecules via 10BASEd-T / NEXT-A Reactions
• Turn-on/keep-on fluctuated fluorescent molecules as targeted binders
• 小・中・高分子、何でもアリの分子標的型薬剤/医用材料開発
• Chemoenzymatic synthesis of biologics-fused hybrid molecules via NEXT-A reaction
• Turn-on / keep-on fluorescent molecules as targeted binders
• Combinatorially Screened Peptide as Targeted Covalent Binder
• 生体系と人工系の良いとこ取りをしたネオバイオ分子の創成と実験医学へ展開
• 難病を制する創薬に活用できるI科の技術についてお話ししよう
• 創薬を指向したT7ファージウィルス上でのネオバイオ分子の創成
• Engineering Neobiologics for Drug-Discovery
• Creation of neobiological molecules via the 10BASEd-T for drug discovery
• 生体系と人工系の良いとこ取りをしたネオバイオ分子の創成と実験医学への展開 [Othres link]
• 創薬を指向したT7ファージウィルス上でのネオバイオ分子の創成と LC/MSによる反応解析 [Othres link]
• Turn-on and Color-changeable Fluorogenic Molecular Probe for Specific Protein Detection Created by the 10BASEd-T [Othres link]
• Turn-on and color-changeable fluorogenic sensor created by the 10BASEd-T
• Artificial Molecule Evolution via the 10BASEd-T
• 創薬・診断を目指した10BASEd-T法の応用：ファージ上での人工分子の共進化
• 人工分子コアの10BASEd-T法による進化
• Evolution of artificial molecule core via the 10BASEd-T
• 進化分子工学（10BASEd-T法）による人工抗体代替物の創成
• Cryptand library construction via the 10BASEd-T for specific binding toward a cancer-related protein
• Turn-on and color-changeable fluorogenic sensor created by the 10BASEd-T
• 人工分子の進化によるものづくり（創薬システム工学を中心に）
• 10BASEd-T法による人工分子コアの進化
• Construction of peptide/protein-hybrid molecules via the NEXT-A and the 10BASEd-T reaction for PET imaging
• Artificial Molecule Evolution via the 10BASEd-T
• Drug-discovery systems engineering: construction of peptide/protein hybrid molecules via the NEXT-A and/or the 10BASEd-T reaction
• Cancer detection and cure: antibody drugs and (hybrid) peptides as antibody substitutes
• Position-specific modification of nucleic acids/peptides/proteins toward pharmaceutical systems engineering
• 創薬システムエンジニアリング：NEXT-A法および10BASEd-T法の開発
• NEXT-A反応による蛋白質/ペプチドの迅速標識
• Introduction of functional amino acids at the N-terminus of peptide/protein by the NEXT-A (N-Terminal EXtension with Transferase and ARS) reaction
• Synthesis of cyclic peptides/proteins using the NEXT-A/Cyclization reaction
• NEXT-A反応を用いた蛋白質N末端への機能性非天然アミノ酸導入
• N-terminal specific 18F-labeling of peptides and proteins
• Synthesis of cyclic peptides using the NEXT-A reaction
• Introduction of functional amino acids at the N-terminus of peptide/protein
• N-TERMINAL SPECIFIC POINT-IMMOBILIZATION OF PEPTIDE/PROTEIN BY THE ONE-POT NEXT-A REACTION
• The NEXT-A reaction
• Tag-Free N-Terminal Specific Immobilization of Lectin via the NEXT-A Reaction for Sugar Detection
• An invited lecture-Regiospecific modifications of (bio)macromolecules
• L/F-transferaseを用いた蛋白質N末端特異的な蛍光および陽電子放射断層撮影(PET)プローブ標識
• Regiospecific modifications of (bio)macromolecules
• 4塩基コドン／アンチコドン対を用いた哺乳動物 生細胞内での遺伝暗号の拡張
• Position-specific incorporation of blue-laser excitable fluorescent amino acids into peptides

書籍等出版物

• Direct combinatorial screening of a target-specific covalent binding peptide: activation of the warhead reactivity in a pseudo-catalytic microenvironment, Peptide Science 2021
• Reversal of covalent conjugation of benzoxaborole-modified targeted peptide by reduction, Peptide Science 2021
• Construct of cyclic peptide phage library containing covalent warhead, Peptide Science 2021
• A solvatochromic binder obtained via an extended phage-display acts as a fluororeporter for the fragment-based drug discovery / Peptide Science 2020
• Chemo-​enzymatic Synthesis of biologics-​fused hybrid molecules via NEXT-​A reaction / Peptide Science 2019
• 「ファージディスプレイ法を利用した機能性ペプチドスクリーニング」, 医薬品開発における中分子領域（核酸医薬・ペプチド医薬）の開発戦略
• Combinatorially Screened Peptide as Targeted Covalent Binder / Peptide Science 2018
• Construction of cryptand library via the Gp10 based-thioetherification (10BASEd-T) / Peptide Science 2016
• Bioconjugation approach towards peptide-Fc fusion compounds / Peptide Science 2016
• Theoretical/Experimental Structural Analysis of Protein-Ligand Interaction between GST and its Fluorogenic Binder Created by the 10BASEd-T / Peptide Science 2015
• Synthesis of Multicyclic Library for Finding Strong Target-Specific Binders / Peptide Science 2014
• Artificial Macrocycle as a Functional-Equivalent of Catalytic Antibody / Peptide Science 2014
• Construction of a Peptide Expression Vector Library for Phenotypic Screening of Bioactive Peptides in Yeast / Peptide Science 2013
• Selection of Streptavidin-Binding Artificial Peptide Posessing Salicylic Acid Moiety via the 10BASEd-T on the Bacteriophage T7 / Peptide Science 2013
• An Artificial Molecule-Peptide Hybrid Discovered via the 10BASEd-T Binds to the Substrate-Binding Site of Glutathione S-Transferase / Peptide Science 2013
• Synthesis of N-terminal specific PET-probe labeling of peptides via the NEXT-A reaction / Peptide Science 2012
• 第2章「創薬システムエンジニアリング：NEXT-A法および10BASEd-T法の開発」, 総合コミュニケーション科学シリーズ ユニーク＆エキサイティング サイエンス２
• Synthesis of cyclic peptides using the NEXT-A Reaction / Peptide Science 2010
• 第4章「L/F-転移酵素による機能性非天然アミノ酸の蛋白質N末端への導入」酵素利用技術大系
• N-Terminal Specific Point-Immobilization and Fluorescence Labeling of Peptide/Protein by the One-Pot NEXT-A Reaction / Peptide Science 2009
• 第2章「タンパク質工学の基礎」バイオプロセスハンドブック
• Position-specific Incorporation of Blue-laser Excitable Fluorescent Amino Acids into Peptides and Proteins / Peptide Science 2005
• Spectroscopic properties of some [60]fullerene-o-quinodimethane monoadducts and bisadducts

作品等

共同研究・競争的資金等の研究課題

• T型／H型ペプチド構造を持つコバレントバインダーによる標的蛋白質の不可逆的阻害
• 血液凝固機能を阻害する非天然型DNAアプタマー薬剤の構造とヌクレアーゼ耐性機構
• 表面プラズモン共鳴小型半導体化学量センサの実用検証補助事業
• 半導体SPRセンサによるコロナウイルスのリアルタイム分布可視化技術開発
• ウイルス可視化のためのプラズモニック半導体センサ
• 蛍光分子の2段階成熟による環境応答性センサー型分子の取得
• 蛋白質生合成系の有機化学的拡張と合成生命体の創成

メディア報道

産業財産権

• 中和可能コバレントドラッグ
• マクロ環分子およびそのスクリーニング方法
• ペプチド又はタンパク質へのフッ素含有アミノ酸導入法
• ペプチド又はタンパク質へのフッ素導入法
• 目的タンパク質または目的ペプチドにアミノ酸を導入する方法
• 化学修飾されたペプチドを有するファージおよびその製造方法
• ダンベル型DNAの効率的な製造方法
• 蛍光性アミノ酸誘導体
• 新規蛍光性アミノ酸誘導体およびその製造方法

所属学協会

• 総合コミュニケーション科学学会
• 日本化学会（生体機能関連化学部会等も含む）
• 日本ペプチド学会

委員歴

担当経験のある科目

• 化学生命工学実験第二
• 生物学
• 現代化学
• 有機化学第一
• 化学生命工学演習第一
• 生物有機化学
• 生体分子化学特論
• UECパスポートセミナー2021
• 生物有機化学
• 基盤理工学専攻基礎

社会貢献活動

• 機械学習セミナー
• 中分子共有結合薬剤の基礎開発と医工産学連携の実例

学術貢献活動

• 量子科学研究センター
• セッションチェア＠核酸関連（第17回バイオ関連化学シンポジウム）